Speckle correlation resolution enhancement of wide-field fluorescence imaging
نویسندگان
چکیده
HASAN YILMAZ,* ELBERT G. VAN PUTTEN, JACOPO BERTOLOTTI, AD LAGENDIJK, WILLEM L. VOS, AND ALLARD P. MOSK Complex Photonic Systems (COPS), MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands Currently address: Philips Research Laboratories, 5656 AE Eindhoven, The Netherlands Currently address: Physics and Astronomy Department, University of Exeter, Stocker Road, Exeter EX4 4QL, UK *Corresponding author: [email protected]
منابع مشابه
Wide-field fluorescence sectioning with hybrid speckle and uniform-illumination microscopy.
We describe a method of obtaining optical sectioning with a standard wide-field fluorescence microscope. The method involves acquiring two images, one with nonuniform illumination (in our case, speckle) and another with uniform illumination (in our case, randomized speckle). An evaluation of the local contrast in the speckle-illumination image provides an optically sectioned image with low reso...
متن کامل13-fold resolution gain through turbid layer via translated unknown speckle illumination.
Fluorescence imaging through a turbid layer holds great promise for various biophotonics applications. Conventional wavefront shaping techniques aim to create and scan a focus spot through the turbid layer. Finding the correct input wavefront without direct access to the target plane remains a critical challenge. In this paper, we explore a new strategy for imaging through turbid layer with a l...
متن کاملWide-field extended-resolution fluorescence microscopy with standing surface-plasmon-resonance waves.
The resolution of conventional surface-plasmon-resonance (SPR) imaging has been limited by the diffraction nature of light. A wide-field extended-resolution optical imaging technique, standing-wave SPR fluorescence (SW-SPRF) microscopy, has been developed. Based on evanescent SPR standing waves, SW-SPRF provides lateral resolution approaching 100 nm and offers the advantages of significant sign...
متن کاملContrast-enhanced imaging of cerebral vasculature with laser speckle.
High-resolution cerebral vasculature imaging has applications ranging from intraoperative procedures to basic neuroscience research. Laser speckle, with spatial contrast processing, has recently been used to map cerebral blood flow. We present an application of the technique using temporal contrast processing to image cerebral vascular structures with a field of view a few millimeters across an...
متن کاملNon-diffractive computational ghost imaging.
Computational ghost imaging (CGI) enables an image to be recorded using a single-pixel detector. The image can be reconstructed from correlations between the scene and a series of known projected intensity patterns. In this work we investigate the performance of CGI using pseudo non-diffracting (ND) speckle patterns. We demonstrate an extended depth-of-field that is ∼ 2-3 times greater than tha...
متن کامل